Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model
3,356 Views
•
06:05 min
•
March 09, 2022
DOI:
Özet
Automatically generated
Transmitochondrial cybrids are hybrid cells obtained by fusing mitochondrial DNA (mtDNA)-depleted cells (rho0 cells) with cytoplasts (enucleated cells) derived from patients affected by mitochondrial disorders. They allow the determination of the nuclear or mitochondrial origin of the disease, evaluation of biochemical activity, and confirmation of the pathogenetic role of mtDNA-related variants.
Read Article
Cite this Article
Cavaliere, A., Marchet, S., Di Meo, I., Tiranti, V. An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model. J. Vis. Exp. (181), e63452, doi:10.3791/63452 (2022).
Copy