Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / An In Vitro Approach to Study Mitochondrial…
JoVE Journal
Genetics

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Genetics
An In Vitro Approach to Study Mitochondrial Dysfunction
 

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Article DOI: 10.3791/63452-v • 06:05 min • March 9th, 2022
March 9th, 2022 •
*1, *1, 1, 1
1Unit of Medical Genetics and Neurogenetics, Fondazione IRCCS Istituto Neurologico Carlo Besta
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Transmitochondrial cybrids are hybrid cells obtained by fusing mitochondrial DNA (mtDNA)-depleted cells (rho0 cells) with cytoplasts (enucleated cells) derived from patients affected by mitochondrial disorders. They allow the determination of the nuclear or mitochondrial origin of the disease, evaluation of biochemical activity, and confirmation of the pathogenetic role of mtDNA-related variants.

Tags

In Vitro Approach Mitochondrial Dysfunction Cybrid Model Mitochondrial DNA Mutation Atriplasmy Severity Of The Disease Biochemical Investigation Nuclear Background Pathogenicity Impact At Biochemical Level 35 Millimeter Dishes Fibroblasts 1X PBS Calcium And Magnesium 70% Ethanol Nucleation Medium Bubbles Centrifuge Bottle Centrifuge 37 Degrees Celsius 8,000 X G
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter