Dergi
/
Nörobilim
/
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

DOI:
10.3791/63386-v

•

10:37 min

•

January 21, 2022

•

, ,
1Biyokimya ve Moleküler Biyoloji Anabilim Dalı,State University of New York Upstate Medical University, 2Department of Neurology,State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Bölümler

  • 00:04Introduction
  • 00:46Construction of Perfusion Apparatus and Dissection Platform
  • 02:40Preparation of the Paraformaldehyde Solution
  • 04:58Transcardial “Pump-Free” Perfusion
  • 09:18Results: Histological Analysis Following Gravity-Fed Perfusion
  • 10:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

Etiketler

Gravity-fed Transcardial PerfusionHistologic AnalysisMouse Central Nervous SystemFixative PerfusionProtocolApparatusBuffer BottlesMicro SpatulasTubingSyringePBSPFAStyrofoam Block

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster

Read Article