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A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

6,965 Views

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10:37 min

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January 21, 2022

DOI:

10.3791/63386-v

DOI:
10.3791/63386-v

•

10:37 min
January 21, 2022

•

30 Views
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1Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University, 2Department of Neurology,State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Summary

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A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

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Rana, A., Massa, P. T., Chen, X. J. A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System. J. Vis. Exp. (179), e63386, doi:10.3791/63386 (2022).

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