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定量微管分离技术分离小鼠组织中稳定的微管、不稳定的微管和游离微管蛋白
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JoVE Journal Biyokimya
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
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定量微管分离技术分离小鼠组织中稳定的微管、不稳定的微管和游离微管蛋白

1,556 Views

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07:21 min

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November 17, 2023

DOI:

10.3791/63358-v

DOI:
10.3791/63358-v

•

07:21 min
November 17, 2023

•

24 Views
,
1Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, 2Center for Research in Neurodegenerative Diseases,Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

Özet

Automatically generated

微管是微管蛋白聚合物，在真核细胞中作为细胞骨架成分起着至关重要的作用，并以其动态不稳定性而闻名。本研究开发了一种将微管分离为稳定微管、不稳定微管和游离微管蛋白的方法，以评估微管在各种小鼠组织中的稳定性。

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Keywords Extracted From The Given Text:Quantitative Microtubule FractionationStable MicrotubulesLabile MicrotubulesFree TubulinMicrotubule StabilityAlzheimer’s DiseaseCancerPhosphate Buffer SolutionPBSMicrotubule Stabilizing BufferMSBCentrifugationSupernatantPrecipitateSodium Dodecyl SulfateSDS Sample BufferWestern Blotting

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Hagita-Tatsumoto, A., Miyasaka, T. Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues. J. Vis. Exp. (201), e63358, doi:10.3791/63358 (2023).

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