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Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
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Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
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October 19, 2021
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An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.
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Aguilera-Castrejon, A., Hanna, J. H. Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis. J. Vis. Exp. (176), e63160, doi:10.3791/63160 (2021).
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