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Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
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Capitoli
- 00:04Introduction
- 00:47Ex Utero Roller Culture of Embryos from Embryonic Day 7.5 to 11
- 03:48Extended Embryo Culture from Embryonic Day 5.5/6.5 to 11
- 04:38Results: Continuous and Robust Ex Utero Development of Post Implantation Mouse Embryos
- 06:34Conclusion
An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.
