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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Biyomühendislik
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

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12:26 min

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January 29, 2022

DOI:

10.3791/63121-v

DOI:
10.3791/63121-v

•

12:26 min
January 29, 2022

•

8 Views
, , , , , , ,
1Department of Cellular Biophysics,Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant,Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE),Heidelberg University

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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

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Christian, J., Blumberg, J. W., Probst, D., Lo Giudice, C., Sindt, S., Selhuber-Unkel, C., Schwarz, U. S., Cavalcanti-Adam, E. A. Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy. J. Vis. Exp. (179), e63121, doi:10.3791/63121 (2022).

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