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产生来自多能干细胞的自组装人心脏类器官
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JoVE Journal Biyokimya
Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
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产生来自多能干细胞的自组装人心脏类器官

DOI:
10.3791/63097-v

•

08:56 min

•

September 15, 2021

•

, , , ,
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, 2Department of Biomedical Engineering, College of Engineering,Michigan State University

Bölümler

  • 00:04Introduction
  • 00:59Generation of 3D Self-Assembling Human Heart Organoids
  • 02:51Human Heart Organoid (hHO) Differentiation
  • 05:33Organoid Analysis
  • 06:03Results: Self-organizing hHO
  • 08:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里，我们描述了一种方案，通过自组织有效地使用人类多能干细胞来创建与发育相关的人类心脏类器官（hHO）。该协议依赖于发育线索的顺序激活，并产生高度复杂，功能相关的人体心脏组织。

Etiketler

Self-assemblingHuman HeartOrganoidsPluripotent Stem Cells3D StructureDeveloping HeartCardiovascular DiseasesPharmacological TreatmentsHigh ThroughputCongenital Heart DefectsEmbryoid BodiesCell CultureDifferentiationHPSCDPBSACUTAPSC MediumThiasCell SuspensionCell CounterHemo CytometerRound Bottom Ultra Low Attachment 96 Well PlateRPMI 1640Insulin Free B27 Supplement

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