Dergi
/
Biyoloji
/
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

2,348 Views

•

09:07 min

•

September 20, 2021

DOI:

10.3791/62968-v

DOI:
10.3791/62968-v

•

09:07 min
September 20, 2021

•

5 Views
, , ,
1Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Özet

Automatically generated

This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity.

Explore More Videos

Live-cell ImagingTranscriptional ActivityDNA Double-strand BreaksTranscription DetectionSingle-cell AnalysisDNA Damage ResponseTranscription-replication CrosstalkDoxycycline InductionCalibration MeasurementsMicrocentrifuge Tube PreparationTransfectionGlass Bottom DishMicroscopy Observation

Read Article

Cite this Article

de Almeida, M. R., Gameiro, E., de Almeida, S. F., Martin, R. M. Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks. J. Vis. Exp. (175), e62968, doi:10.3791/62968 (2021).

Download .ris file

Copy

Share Video

.

Copy