Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Madalena R. de Almeida; Eduardo Gameiro; S&#233;rgio F. de Almeida; Robert  M. Martin

doi:10.3791/62968

Journal

/

Biología

/

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

2,348 Views

•

09:07 min

•

September 20, 2021

DOI:

10.3791/62968-v

DOI:

10.3791/62968-v

•

09:07 min

September 20, 2021

•

5 Views

Madalena R. de Almeida¹, Eduardo Gameiro¹, Sérgio F. de Almeida¹, Robert M. Martin¹

¹Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Summary

This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity.

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Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

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