Dergi
/
Gelişim Biyolojisi
/
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

2,341 Views

•

08:16 min

•

August 13, 2021

DOI:

10.3791/62930-v

DOI:
10.3791/62930-v

•

08:16 min
August 13, 2021

•

10 Views
, , ,
1Biyoloji Bölümü,Indiana University of Pennsylvania

Özet

Automatically generated

The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development.

Explore More Videos

In Situ HybridizationZebrafish LarvaeZebrafish JuvenilesMesonephros DevelopmentGene ExpressionProbe PenetrationKidney VisualizationMatingEmbryo CollectionLarva FixationLarva DehydrationLarva RehydrationProteinase K DigestionBleaching

Read Article

Cite this Article

Kasar, S. N., Grandinette, S. A., Semelsberger, S. D., Diep, C. Q. In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development. J. Vis. Exp. (174), e62930, doi:10.3791/62930 (2021).

Download .ris file

Copy

Share Video

.

Copy