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Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
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Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
Bölümler
- 00:05Introduction
- 01:26Antigen Preparation for Subcutaneous Injection
- 02:31Subcutaneous Injection
- 03:31Intravitreal Injection Procedure
- 05:57Optical Coherence Tomography (OCT) Imaging to Detect and Quantify Uveitis
- 07:35Results: Primed Mycobacterial Uveitis Model for Induction of Uveitis in Mice
- 09:12Conclusion
This protocol outlines the steps for inducing Primed Mycobacterial Uveitis (PMU) in mice. This method outlines the steps to help produce reliable and robust ocular inflammation in the mouse model system. Using this protocol, we generated uveitic eyes and uninflamed fellow eyes from single animals for further evaluation with immunologic, transcriptomic, and proteomic assays.
