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Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

2,357 Views

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10:33 min

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December 17, 2021

DOI:

10.3791/62925-v

DOI:
10.3791/62925-v

•

10:33 min
December 17, 2021

•

7 Views
, , , ,
1Department of Ophthalmology,University of Washington, 2Academic Unit of Ophthalmology, Translational Health Sciences,University of Bristol

Summary

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This protocol outlines the steps for inducing Primed Mycobacterial Uveitis (PMU) in mice. This method outlines the steps to help produce reliable and robust ocular inflammation in the mouse model system. Using this protocol, we generated uveitic eyes and uninflamed fellow eyes from single animals for further evaluation with immunologic, transcriptomic, and proteomic assays.

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John, S., Bell, O. H., Wilson, L., Copland, D. A., Pepple, K. L. Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis. J. Vis. Exp. (178), e62925, doi:10.3791/62925 (2021).

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