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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
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September 20, 2021
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1Department of Nephrology and Medical Intensive Care Medicine,Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
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Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.
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Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K. M. Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing. J. Vis. Exp. (175), e62901, doi:10.3791/62901 (2021).
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