This content is Free Access.
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
3,861 Views
•
06:00 min
•
September 20, 2021
DOI:
1Department of Nephrology and Medical Intensive Care Medicine,Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Summary
Automatically generated
Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.
Read Article
Cite this Article
Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K. M. Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing. J. Vis. Exp. (175), e62901, doi:10.3791/62901 (2021).
Copy