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毛细管电泳电喷雾电离质谱法绝对定量肌醇焦磷酸盐
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Biyokimya
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JoVE Journal Biyokimya
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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毛细管电泳电喷雾电离质谱法绝对定量肌醇焦磷酸盐

DOI:
10.3791/62847-v

•

09:22 min

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August 13, 2021

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, , ,
1Institute of Organic Chemistry,University of Freiburg, 2Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Bölümler

  • 00:04Introduction
  • 00:45Setting Up the Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) System
  • 01:38Preparation of Buffer, Capillary, and CE-MS System
  • 03:28Performing the CE-ESI-MS Runs
  • 05:10Data Analysis
  • 06:50Results: Quantitation of Inositol Pyrophosphates from Mammalian Cell Extracts by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
  • 08:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

描述了毛细管电泳电喷雾电离质谱法用于绝对定量哺乳动物细胞提取物中肌醇焦磷酸盐的程序。

Etiketler

Inositol PyrophosphatesCapillary ElectrophoresisElectrospray IonizationMass SpectrometryCE-ESI-MSInositol Phosphate MetabolismQuantitationBiosynthetic PathwayCE Running BufferSheath LiquidCapillary ColumnESI SprayerInositol Phosphate Standards

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