Journal
/
生物化学
/
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

1,903 Views

•

09:22 min

•

August 13, 2021

DOI:

10.3791/62847-v

DOI:
10.3791/62847-v

•

09:22 min
August 13, 2021

•

9 Views
, , ,
1Institute of Organic Chemistry,University of Freiburg, 2Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Summary

Automatically generated

A procedure for capillary electrophoresis electrospray ionization mass spectrometry for the absolute quantitation of inositol pyrophosphates from mammalian cell extracts is described.

Explore More Videos

Inositol PyrophosphatesCapillary ElectrophoresisElectrospray IonizationMass SpectrometryCE-ESI-MSInositol Phosphate MetabolismQuantitationBiosynthetic PathwayCE Running BufferSheath LiquidCapillary ColumnESI SprayerInositol Phosphate Standards

Read Article

Cite this Article

Qiu, D., Eisenbeis, V. B., Saiardi, A., Jessen, H. J. Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry. J. Vis. Exp. (174), e62847, doi:10.3791/62847 (2021).

Download .ris file

Copy

Share Video

.

Copy