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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Biochimie
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Journal JoVE Biochimie
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

1,903 Views

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09:22 min

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August 13, 2021

DOI:

10.3791/62847-v

DOI:
10.3791/62847-v

•

09:22 min
August 13, 2021

•

9 Views
, , ,
1Institute of Organic Chemistry,University of Freiburg, 2Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Summary

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A procedure for capillary electrophoresis electrospray ionization mass spectrometry for the absolute quantitation of inositol pyrophosphates from mammalian cell extracts is described.

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Cite this Article

Qiu, D., Eisenbeis, V. B., Saiardi, A., Jessen, H. J. Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry. J. Vis. Exp. (174), e62847, doi:10.3791/62847 (2021).

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