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Chirurgie et traitement des échantillons pour l’imagerie corrélative de la valve pulmonaire murine
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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
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Chirurgie et traitement des échantillons pour l’imagerie corrélative de la valve pulmonaire murine

2,416 Views

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07:34 min

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August 05, 2021

DOI:

10.3791/62581-v

DOI:
10.3791/62581-v

•

07:34 min
August 05, 2021

•

1 Views
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1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

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Ici, nous décrivons un flux de travail corrélatif pour l’excision, la pressurisation, la fixation et l’imagerie de la valve pulmonaire murine afin de déterminer la conformation grossière et les structures de la matrice extracellulaire locale.

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Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

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Liu, Y., Lee, Y., Yi, T., Wu, K., Bouchet-Marquis, C., Chan, H., Breuer, C. K., McComb, D. W. Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve. J. Vis. Exp. (174), e62581, doi:10.3791/62581 (2021).

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