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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

DOI:
10.3791/62510-v

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08:57 min

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July 17, 2021

•

,
1Structural Genomics Consortium,University of Toronto

Bölümler

  • 00:00Introduciton
  • 00:28Generation of a Recombinant Baculovirus Stocks
  • 03:12Small-scale Proteins Expression Screening and Virus Amplificaiton
  • 05:26Infection of the Sf9 with SCBIIC for Protein Production
  • 06:09Sonuçlar
  • 06:51Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The baculovirus expression vector system (BEVS) is a robust platform for expression screening and production of protein arginine methyltransferases (PRMTs) to be used for biochemical, biophysical, and structural studies. Milligram quantities of material can be produced for the majority of PRMTs and other proteins of interest requiring a eukaryotic expression platform.

Etiketler

Recombinant PRMT ProteinsBaculovirus Expression Vector SystemProtein ExpressionSF9 CellsTransfectionSerum-free Insect MediaProtein PurificationBiochemical StudiesBiophysical StudiesStructural Studies

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