Dergi
/
Biyokimya
/
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

2,015 Views

•

09:40 min

•

April 28, 2022

DOI:

10.3791/62409-v

DOI:
10.3791/62409-v

•

09:40 min
April 28, 2022

•

2 Views
, , ,
1Department of Experimental Oncology,European Institute of Oncology IRCCS (IEO), 2European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO

Özet

Automatically generated

Protein Arginine (R)-methylation is a wide-spread post-translational modification regulating multiple biological pathways. Mass spectrometry is the best technology to globally profile the R-methyl-proteome, when coupled to biochemical approaches for modified peptide enrichment. The workflow designed for the high confidence identification of global R-methylation in human cells is described here.

Explore More Videos

Mass SpectrometryProteomicsProtein R-methylationPRMT InhibitorsDTTIodoacetamideTrypsinLysargiNaseHigh PH Reversed Phase Liquid ChromatographyImmuno-affinity Enrichment

Read Article

Cite this Article

Maniaci, M., Marini, F., Massignani, E., Bonaldi, T. A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis. J. Vis. Exp. (182), e62409, doi:10.3791/62409 (2022).

Download .ris file

Copy

Share Video

.

Copy