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A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
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生物化学
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JoVE 杂志 生物化学
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

1,989 Views

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09:40 min

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April 28, 2022

DOI:

10.3791/62409-v

DOI:
10.3791/62409-v

•

09:40 min
April 28, 2022

•

3 Views
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1Department of Experimental Oncology,European Institute of Oncology IRCCS (IEO), 2European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO

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Mass SpectrometryProteomicsProtein R-methylationPRMT InhibitorsDTTIodoacetamideTrypsinLysargiNaseHigh PH Reversed Phase Liquid ChromatographyImmuno-affinity Enrichment

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Maniaci, M., Marini, F., Massignani, E., Bonaldi, T. A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis. J. Vis. Exp. (182), e62409, doi:10.3791/62409 (2022).

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