Presented here is a simple protocol for the directed differentiation of hemogenic endothelial cells from human pluripotent stem cells in approximately 1 week.
Nelson, E. A., Qiu, J., Chavkin, N. W., Hirschi, K. K. Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (169), e62391, doi:10.3791/62391 (2021).