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从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动

4,758 Views

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06:53 min

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July 10, 2021

DOI:

10.3791/62272-v

DOI:
10.3791/62272-v

•

06:53 min
July 10, 2021

•

8 Views
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1Department of Veterinary Medicine,University of Sassari

Özet

Automatically generated

该议定书旨在为成年和幼羊卵母细胞的玻璃化提供标准方法。它包括从体外成熟介质的准备到暖化后文化的所有步骤。卵母细胞在 MII 阶段使用 Cryotop 进行振动，以确保最低基本体积。

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Cite this Article

Succu, S., Serra, E., Gadau, S., Varcasia, A., Berlinguer, F. Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep. J. Vis. Exp. (173), e62272, doi:10.3791/62272 (2021).

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