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Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging
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JoVE Journal Nörobilim
Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

8,710 Views

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11:57 min

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February 24, 2021

DOI:

10.3791/62267-v

DOI:
10.3791/62267-v

•

11:57 min
February 24, 2021

•

63 Views
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1Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS,PSL Research University, 2Institute of Psychiatry and Neurosciences of Paris, INSERM U1266,Université de Paris, 3Iconeus, 4Department of Pharmacology and Toxicology,Jena University Hospital – Friedrich Schiller University Jena

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This protocol describes the quantification of volumetric cerebral hemodynamic variations in the mouse brain using functional ultrasound (fUS). Procedures for 3D functional activation map following sensory stimulation as well as resting-state functional connectivity are provided as illustrative examples, in anesthetized and awake mice.

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Bertolo, A., Nouhoum, M., Cazzanelli, S., Ferrier, J., Mariani, J., Kliewer, A., Belliard, B., Osmanski, B., Deffieux, T., Pezet, S., Lenkei, Z., Tanter, M. Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging. J. Vis. Exp. (168), e62267, doi:10.3791/62267 (2021).

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