Cryo-EM and Single-Particle Analysis with Scipion
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May 29, 2021
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Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological specimen.
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Jiménez-Moreno, A., del Caño, L., Martínez, M., Ramírez-Aportela, E., Cuervo, A., Melero, R., Sánchez-García, R., Strelak, D., Fernández-Giménez, E., de Isidro-Gómez, F., Herreros, D., Conesa, P., Fonseca, Y., Maluenda, D., Jiménez de la Morena, J., Macías, J., Losana, P., Marabini, R., Carazo, J., Sorzano, C. Cryo-EM and Single-Particle Analysis with Scipion. J. Vis. Exp. (171), e62261, doi:10.3791/62261 (2021).
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