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Cryo-EM and Single-Particle Analysis with Scipion
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Cryo-EM and Single-Particle Analysis with Scipion
DOI:

09:06 min

May 29, 2021

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Chapters

  • 00:05Introduction
  • 00:53Project Creation and Data Import
  • 01:42Movie Alignment: From Movies to Micrographs
  • 02:37Particle Picking
  • 05:28Evaluation and Post-Processing
  • 06:53Results: Representative Single-Particle Analysis
  • 08:26Conclusion

Summary

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Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological specimen.

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