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体外荧光显微镜基于动感检测的肌素特异性适应
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JoVE Journal Biyokimya
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
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体外荧光显微镜基于动感检测的肌素特异性适应

5,406 Views

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08:57 min

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February 04, 2021

DOI:

10.3791/62180-v

DOI:
10.3791/62180-v

•

08:57 min
February 04, 2021

•

9 Views
, , , ,
1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute,National Institutes of Health, 2Department of Physiology, Perelman School of Medicine,University of Pennsylvania

Özet

Automatically generated

这里介绍的是一个过程来表达和净化肌素5a，然后讨论其特征，使用合奏和单分子体外荧光显微镜为基础的测定，以及如何修改这些方法来描述非肌肉肌素2b。

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Cite this Article

Tripathi, A., Bond, C., Sellers, J. R., Billington, N., Takagi, Y. Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays. J. Vis. Exp. (168), e62180, doi:10.3791/62180 (2021).

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