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来自肠道密码的穆林原结肠上皮单层的生成
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
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来自肠道密码的穆林原结肠上皮单层的生成

6,599 Views

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06:57 min

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February 06, 2021

DOI:

10.3791/62156-v

DOI:
10.3791/62156-v

•

06:57 min
February 06, 2021

•

30 Views
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1Department of Pathology, School of Medicine,University of Michigan

Özet

Automatically generated

在此协议中，我们描述了如何直接从肠道密码生成穆林原发性上皮结肠单层。我们提供实验方法，在透气过滤器上生成汇流单层，在划痕伤口愈合和生化研究中产生汇流单层，以及用于免疫荧光分析的稀疏和共生单层。

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Cite this Article

Muraleedharan, C. K., Mierzwiak, J., Feier, D., Nusrat, A., Quiros, M. Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts. J. Vis. Exp. (168), e62156, doi:10.3791/62156 (2021).

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