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Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
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Biologia
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JoVE Journal Biologia
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

6,332 Views

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06:57 min

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February 06, 2021

DOI:

10.3791/62156-v

DOI:
10.3791/62156-v

•

06:57 min
February 06, 2021

•

12 Views
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1Department of Pathology, School of Medicine,University of Michigan

Tags

Primary Colon Epithelial MonolayersIntestinal CryptsWound RepairPermeability BarrierTransepithelial MigrationHost-pathogen InteractionDamaged EpithelialDrug DiscoveryColon DissectionCell IsolationEDTACell Culture

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Muraleedharan, C. K., Mierzwiak, J., Feier, D., Nusrat, A., Quiros, M. Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts. J. Vis. Exp. (168), e62156, doi:10.3791/62156 (2021).

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