This content is Free Access.
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
6,518 Views
•
06:57 min
•
February 06, 2021
DOI:
Summary
Automatically generated
In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.
Read Article
Cite this Article
Muraleedharan, C. K., Mierzwiak, J., Feier, D., Nusrat, A., Quiros, M. Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts. J. Vis. Exp. (168), e62156, doi:10.3791/62156 (2021).
Copy