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Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
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Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Bölümler
- 00:05Introduction
- 00:38Embryonic Palatal Shelf Dissection
- 02:54Mouse Embryonic Palatal Mesenchymal (MEPM) Cell Culture
- 04:072D Collective Migration Assay Setup
- 04:52Wound Repair Assay Setup
- 05:28Results: Representative MEPM Imaging and Collective Movement Analysis
- 06:33Conclusion
We present a protocol for isolation and culture of primary mouse embryonic palatal mesenchymal cells for time-lapse imaging of two-dimensional (2D) growth and wound-repair assays. We also provide the methodology for analysis of the time-lapse imaging data to determine cell-stream formation and directional motility.
