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Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
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发育生物学
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JoVE 杂志 发育生物学
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

DOI:
10.3791/62151-v

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07:13 min

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February 13, 2021

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, , ,
1Department of Anatomy and Cell Biology,University of Kansas Medical Center, 2Department of Biological Physics,Eotvos University

Chapters

  • 00:05Introduction
  • 00:38Embryonic Palatal Shelf Dissection
  • 02:54Mouse Embryonic Palatal Mesenchymal (MEPM) Cell Culture
  • 04:072D Collective Migration Assay Setup
  • 04:52Wound Repair Assay Setup
  • 05:28Results: Representative MEPM Imaging and Collective Movement Analysis
  • 06:33Conclusion

Summary

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We present a protocol for isolation and culture of primary mouse embryonic palatal mesenchymal cells for time-lapse imaging of two-dimensional (2D) growth and wound-repair assays. We also provide the methodology for analysis of the time-lapse imaging data to determine cell-stream formation and directional motility.

Tags

Primary Mouse Embryonic Palatal Mesenchyme CellsTime-lapse ImagingCollective Movement AttributesPalatal Shelf ElevationCraniofacial FieldMesenchymal RemodelingEmbryonic Day 13.5Palatal ShellMEPM Culture MediumFine ForcepsStainless Steel ScissorsCell Isolation

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