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优化了炔基脂肪酸类似物的掺入，用于使用点击化学检测脂肪酰化蛋白
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
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优化了炔基脂肪酸类似物的掺入，用于使用点击化学检测脂肪酰化蛋白

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:
10.3791/62107-v

•

07:27 min
April 09, 2021

•

28 Views
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1Biyoloji Bölümü,University of Waterloo

Özet

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脂肪酰化的研究在细胞蛋白质相互作用和疾病中具有重要意义。这里介绍的是一种改进的方案，用于改善脂肪酰化蛋白的点击化学检测，其可应用于各种细胞类型并与其他测定相结合，包括脉冲追逐和质谱分析。

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Cite this Article

Liao, L. M. Q., Gray, R. A. V., Martin, D. D. O. Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry. J. Vis. Exp. (170), e62107, doi:10.3791/62107 (2021).

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