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Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

DOI:
10.3791/62086-v

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12:59 min

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February 28, 2021

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, , ,
1Instituto Gulbenkian de Ciência, 2Faculdade de Ciências da Universidade de Lisboa, 3NOVA School of Science and Technology

Bölümler

  • 00:04Introduction
  • 01:04Sample Preparation for Live Imaging
  • 01:57Sample Preparation for Immunofluorescence Microscopy
  • 04:20Repositioning of Embryo to an Anatomically Standard Position Using Fiji/ImageJ
  • 07:19Sample Preparation for Optical Projection Tomography
  • 10:29Results: Visualization and Analysis of Three and Four-Dimensional Image Data of Mouse Embryos
  • 12:23Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

Etiketler

3D Visualization4D VisualizationAxial ElongationSegmentationVertebrate DevelopmentMouse EmbryoLive ImagingImmunofluorescenceCongenital MalformationScoliosisIn Vitro Model

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