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Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
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JoVE Journal 発生生物学
Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

3,423 Views

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12:59 min

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February 28, 2021

DOI:

10.3791/62086-v

DOI:
10.3791/62086-v

•

12:59 min
February 28, 2021

•

5 Views
, , ,
1Instituto Gulbenkian de Ciência, 2Faculdade de Ciências da Universidade de Lisboa, 3NOVA School of Science and Technology

概要

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Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

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Cite this Article

Dias, A., Martins, G. G., Lopes, A., Mallo, M. Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation. J. Vis. Exp. (168), e62086, doi:10.3791/62086 (2021).

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