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Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

6,967 Views

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11:35 min

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April 18, 2021

DOI:

10.3791/62027-v

DOI:
10.3791/62027-v

•

11:35 min
April 18, 2021

•

28 Views
, , , , ,
1College of Life Sciences, Northwest Normal University, 2State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences, 3Department of Bioscience,Tokyo University of Agriculture

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Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.

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Follicular CellsOocytesOvarian FolliclesZebrafishSeparationDissectionGlass CapillaryIn Vitro FertilizationOvaryOvarian Development

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Wang, W., Kang, T., Bai, L., Hu, W., Obata, Y., Li, J. Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish. J. Vis. Exp. (170), e62027, doi:10.3791/62027 (2021).

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