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发育生物学
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Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

7,005 Views

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11:35 min

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April 18, 2021

DOI:

10.3791/62027-v

DOI:
10.3791/62027-v

•

11:35 min
April 18, 2021

•

28 Views
, , , , ,
1College of Life Sciences, Northwest Normal University, 2State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences, 3Department of Bioscience,Tokyo University of Agriculture

Summary

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Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.

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Follicular CellsOocytesOvarian FolliclesZebrafishSeparationDissectionGlass CapillaryIn Vitro FertilizationOvaryOvarian Development

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Cite this Article

Wang, W., Kang, T., Bai, L., Hu, W., Obata, Y., Li, J. Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish. J. Vis. Exp. (170), e62027, doi:10.3791/62027 (2021).

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