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Generación Y Ensamblaje De Nucleocápsides Específicas Del Virus Respiratorio Sincitial
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JoVE Journal Biyokimya
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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Generación Y Ensamblaje De Nucleocápsides Específicas Del Virus Respiratorio Sincitial

3,567 Views

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09:08 min

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July 27, 2021

DOI:

10.3791/62010-v

DOI:
10.3791/62010-v

•

09:08 min
July 27, 2021

•

1 Views
, , , ,
1Department of Biochemistry,Emory University School of Medicine

Özet

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Para el análisis mecánico profundizado de la síntesis sincitial respiratoria del ARN del virus (RSV), divulgamos un protocolo de utilizar la fosfoproteína de la chaperona (p) para el coexpression de la nucleoproteína ARN-libre (N0)para el montaje in vitro subsecuente de las nucleocápsides virus-específicas (NCs).

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Cite this Article

Gao, Y., Ogilvie, C., Raghavan, A., Von Hoffmann, C., Liang, B. Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus. J. Vis. Exp. (173), e62010, doi:10.3791/62010 (2021).

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