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Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

DOI:
10.3791/61872-v

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09:08 min

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October 17, 2020

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, , ,
1Cell and Developmental Biology Department and the Penn Institute for Regenerative Medicine, Perelman School of Medicine,University of Pennsylvania

Bölümler

  • 00:05Introduction
  • 00:46Day-of-Dissection Preparation
  • 02:35Dissection
  • 04:53Imaging
  • 06:46Results: The Live-Imaging Results of the Cultured Gonads, Their Health, and Niche Compaction
  • 08:15Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we provide a dissection protocol required to live-image the late embryonic Drosophila male gonad. This protocol will permit observation of dynamic cellular processes under normal conditions or after transgenic or pharmacological manipulation.

Etiketler

Live ImagingGonadogenesisDissectionDrosophila EmbryoVitelline MembraneRinger’s SolutionHeptane GlueTungsten NeedlePoly-D-LysineCover Slip

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