Here, we provide a dissection protocol required to live-image the late embryonic Drosophila male gonad. This protocol will permit observation of dynamic cellular processes under normal conditions or after transgenic or pharmacological manipulation.
Nelson, K. A., Warder, B. N., DiNardo, S., Anllo, L. Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad. J. Vis. Exp. (164), e61872, doi:10.3791/61872 (2020).