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Flowcytometrische karakterisering van de ontwikkeling van murine B-cellen
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JoVE Journal İmmünoloji ve Enfeksiyon
Flow Cytometric Characterization of Murine B Cell Development
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Flowcytometrische karakterisering van de ontwikkeling van murine B-cellen

12,513 Views

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08:25 min

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January 22, 2021

DOI:

10.3791/61565-v

DOI:
10.3791/61565-v

•

08:25 min
January 22, 2021

•

34 Views
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1Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Özet

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We beschrijven hierin een eenvoudige analyse van de heterogeniteit van het muriene immuun B-celcompartiment in het peritoneum, milt en beenmergweefsel door middel van flowcytometrie. Het protocol kan worden aangepast en uitgebreid naar andere muizenweefsels.

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Cite this Article

Harris, F. M., Meagher, K. A., Zhong, M., Daniel, B. J., Eckersdorff, M., Green, J. A., Voronina, V., Guo, C., Limnander, A., Macdonald, L. E. Flow Cytometric Characterization of Murine B Cell Development. J. Vis. Exp. (167), e61565, doi:10.3791/61565 (2021).

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