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Immunologie et infection
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Flow Cytometric Characterization of Murine B Cell Development
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Immunologie et infection
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Journal JoVE Immunologie et infection
Flow Cytometric Characterization of Murine B Cell Development

Flow Cytometric Characterization of Murine B Cell Development

DOI:
10.3791/61565-v

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08:25 min

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January 22, 2021

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, , , , , , , , ,
1Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Chapitres

  • 00:04Introduction
  • 00:55Stain Cells and Prepare Compensation
  • 05:30Flow Cytometric Data Acquisition and Analysis
  • 06:08Results: Flow Cytometric Analysis for the Characterization of B Cells
  • 07:24Conclusion

Summary

Traduction automatique

Traduction automatique

We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.

Tags

Flow CytometryB Cell DevelopmentMurine ModelsAutoimmunityAntibody TherapeuticsFluorophoresPhenotypingGenetic ManipulationBone MarrowSpleenViability DyeSingle Color Compensation

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