Flow Cytometric Characterization of Murine B Cell Development

Faith  M. Harris; Karoline A. Meagher; Maggie Zhong; Benjamin  J. Daniel; Mark Eckersdorff; Jesse  A. Green; Vera Voronina; Chunguang Guo; Andre Limnander; Lynn  E. Macdonald

doi:10.3791/61565

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Flow Cytometric Characterization of Murine B Cell Development

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İmmünoloji ve Enfeksiyon

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JoVE Journal İmmünoloji ve Enfeksiyon

Flow Cytometric Characterization of Murine B Cell Development

12,554 Views

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08:25 min

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January 22, 2021

DOI:

10.3791/61565-v

DOI:

10.3791/61565-v

•

08:25 min

January 22, 2021

•

34 Views

Faith M. Harris*¹, Karoline A. Meagher*¹, Maggie Zhong¹, Benjamin J. Daniel², Mark Eckersdorff¹, Jesse A. Green¹, Vera Voronina¹, Chunguang Guo¹, Andre Limnander³, Lynn E. Macdonald¹

¹Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., ²Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., ³Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Özet

We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.

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Flow Cytometric Characterization of Murine B Cell Development

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