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Differentiatie en karakterisering van neurale voorlopercellen en neuronen uit embryonale stamcellen van muizen
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Differentiatie en karakterisering van neurale voorlopercellen en neuronen uit embryonale stamcellen van muizen

4,506 Views

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08:47 min

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May 15, 2020

DOI:

10.3791/61446-v

DOI:
10.3791/61446-v

•

08:47 min
May 15, 2020

•

3 Views
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1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Özet

Automatically generated

We beschrijven de procedure voor de in vitro differentiatie van embryonale stamcellen van muizen in neuronale cellen met behulp van de hanging drop-methode. Verder voeren we een uitgebreide fenotypische analyse uit via RT-qPCR, immunofluorescentie, RNA-seq en flowcytometrie.

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Hanafiah, A., Geng, Z., Wang, Q., Gao, Z. Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells. J. Vis. Exp. (159), e61446, doi:10.3791/61446 (2020).

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