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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

6,504 Views

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09:20 min

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August 11, 2020

DOI:

10.3791/61433-v

DOI:
10.3791/61433-v

•

09:20 min
August 11, 2020

•

9 Views
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1Makine Mühendisliği Bölümü,Kentucky Üniversitesi, 2Kimya Bölümü,Kentucky Üniversitesi, 3Fizyoloji Bölümü,Kentucky Üniversitesi, 4UK Light Microscopy Core,Kentucky Üniversitesi

Özet

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Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.

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Cite this Article

Sandin, J. N., Aryal, S. P., Wilkop, T., Richards, C. I., Grady, M. E. Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells. J. Vis. Exp. (162), e61433, doi:10.3791/61433 (2020).

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