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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

6,516 Views

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09:20 min

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August 11, 2020

DOI:

10.3791/61433-v

DOI:
10.3791/61433-v

•

09:20 min
August 11, 2020

•

9 Views
, , , ,
1Department of Mechanical Engineering,University of Kentucky, 2Department of Chemistry,University of Kentucky, 3Department of Physiology,University of Kentucky, 4UK Light Microscopy Core,University of Kentucky

Summary

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Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.

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Cite this Article

Sandin, J. N., Aryal, S. P., Wilkop, T., Richards, C. I., Grady, M. E. Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells. J. Vis. Exp. (162), e61433, doi:10.3791/61433 (2020).

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