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小鸡西里神经神经元的分离与培养
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JoVE Journal Nörobilim
Isolation and Culture of Chick Ciliary Ganglion Neurons
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小鸡西里神经神经元的分离与培养

DOI:
10.3791/61431-v

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14:36 min

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August 08, 2020

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, , , ,
1Institute of Biomedicine, Department of Medical Sciences – iBiMED,University of Aveiro, 2CNC – Center for Neuroscience and Cell Biology,University of Coimbra

Bölümler

  • 00:00Introduction
  • 01:41Preparation of Coverslips
  • 08:00Dissociation and Culture of Chick Ciliary Ganglion Neurons
  • 11:42Representative Results
  • 12:42Discussion and Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

小鸡丝神经通（CG）是寄生虫神经系统的一部分。在神经肌肉相互作用研究中，小鸡CG神经元的神经元培养被证明是有效的细胞模型。我们描述了从小鸡胚胎中解剖、分离和体外培养的详细方案。

Etiketler

Chick Ciliary GanglionCiliary Ganglion NeuronsParasympathetic Nervous SystemCholinergic NeuronsCholinergic SynapsesCiliary NeuronsChoroidal NeuronsNeuromuscular SynapsesCiliary Ganglion DissectionPrimary Cell CultureGlass Coverslip Preparation

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