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Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
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JoVE Journal Nörobilim
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

4,832 Views

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06:53 min

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February 12, 2021

DOI:

10.3791/61411-v

DOI:
10.3791/61411-v

•

06:53 min
February 12, 2021

•

12 Views
,
1School of Basic Medical Sciences,Xi’an Jiaotong University, 2Department of Cell Biology,Duke University, 3Department of Biochemistry,Duke University

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Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

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Cite this Article

Yang, R., Bennett, V. Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments. J. Vis. Exp. (168), e61411, doi:10.3791/61411 (2021).

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